Search results for "TP53 Gene Mutation"

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Analysis of p53 and mdm2 proteins in malignant fibrous histiocytoma in absence of gene alteration: prognostic significance.

2000

TP53 and MDM2 genes and their protein expression were evaluated in frozen and paraffin-embedded tissue from 27 patients with malignant fibrous histiocytoma to elucidate the relationship between them, their implication in tumor progression mechanisms and their possible diagnostic-prognostic value in malignant fibrous histiocytoma. Single-strand conformation polymorphism analysis and direct sequencing of polymerase chain reaction-amplified DNA were used to establish two TP53 mutations (7.4%): a point mutation and a 63-bp duplication. Amplification of the MDM2 gene was observed in two tumors (7.4%) by means of Southern-blot analysis, one of them also carrying the TP53 point mutation. Immunohis…

Tumor suppressor geneBlotting WesternSoft Tissue NeoplasmsBiologyPolymerase Chain ReactionPathology and Forensic MedicineImmunoenzyme TechniquesMiceProto-Oncogene ProteinsGene duplicationGene expressionAnimalsHumansneoplasmsMolecular BiologyGeneTP53 Gene MutationPolymorphism Single-Stranded ConformationalCell NucleusMice Inbred BALB CHistiocytoma Benign FibrousPoint mutationNuclear ProteinsSingle-strand conformation polymorphismProto-Oncogene Proteins c-mdm2Cell BiologyGeneral MedicineDNA NeoplasmMolecular biologyNeoplasm ProteinsSurvival RateBlotting SouthernTumor progressionMutationCancer researchNeoplasm Recurrence LocalTumor Suppressor Protein p53Virchows Archiv : an international journal of pathology
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